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Leptin transgene expression was identified by RT-PCR in VTA or ARC tissue at day 54 following rAAV-leptin injection into the VTA. Top: Leptin RNA (lower band) was identified in the VTA of all rats that were injected with rAAV-leptin into the VTA but not in rAAV-Control injected rats. Bottom: No leptin mRNA was detected in corresponding ARC punches in either rAAV-Control or those injected with rAAV-leptin in the VTA. Upper band represents 18S rRNA as an internal standard.
