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. 2012 Dec 13;6:180. doi: 10.3389/fnins.2012.00180

Figure 6.

Figure 6

Arrestin recruitment and arrestin-dependent desensitization. Cells expressing the receptors shown were incubated with GFP-arrestin and imaged before or after 3 min exposure to TRH. The right panels show TRH-stimulated IP3 production in arrestin-null cells expressing receptor with no arrestin (No Arr), wildtype arrestin 3 (wtArr), or R169E-Arr, which binds activated GPCRs in a phosphorylation-independent fashion. TRH receptors are: Wt, wildtype; 4Ala, Ala substituted for 4 Ser/Thr residues from amino acids 355 through 365; and 4Ala-371Stop, 4Ala receptor truncated at amino acid 370. Even though arrestin binds robustly to receptors lacking phosphosites at amino acids 355–365, arrestin does not desensitize signaling.