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. 2012 Dec 13;2:975. doi: 10.1038/srep00975

Figure 4. Forssman antigen expression after DNA transfection.

Figure 4

(A) Schematic representation of the mouse and human GBGT1 gene products. The mouse and human sequences are schematically shown in dark and light grey, respectively. The restriction enzyme cleavage sites used for the chimera constructions are indicated with and without parentheses for newly created artificial and pre-existed sites, respectively. The amino acid substitutions made are also shown. (B) The mouse-human FS chimeras and their FS activity. The constructs and the results of immunostaining with anti-Forssman antibody are shown. The values are shown in percentage with the expression of the original mouse FS construct and that of a negative control to be 100 and 0, respectively. The experiments were repeated with some constructs. The “ND” stands for “Not determined”. (C) The in vitro mutagenized amino acid substitution constructs and their FS activity. The results of the amino acid substitution constructs are shown.