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Mutant strain verification. (a) Chromosomal regions of genes pykA, pykF, pykA::cat and pykF::aacC1. The annealing sites of the primers employed for strain verification are depicted (green color). (b) Gel electrophoresis of the genomic DNA PCR products of strains VH33 (PTS-), VH34 (PTS-pykA-) and VH35 (PTS-pykA-).
