Table 2.
Active site residues.
| EcIDH | BsIDH | ApIDH | BpIDH | MtIDH1 | HcIDH | YmIDH | PmIDH | TmIDH | TtIDH | AvIDH | CgIDH | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subfamily | I | I | I | I | II | II | II | II | II | IV | M | M |
| Phos loop start | T105 | T96 | T112 | T107 | T78 | T78 | T77 | T78 | T77 | T90 | S86 | S85 |
| Phos loop end | S113 | S104 | S120 | S115 | S95 | S95 | S94 | S95 | S94 | S98 | S132 | S130 |
|
Isocitrate Binding |
N115 | N106 | N122 | N117 | N97 | N97 | N96 | N97 | N96 | N100 | N135 | N133 |
|
Isocitrate Binding |
R119 | R110 | R126 | R121 | R101 | R101 | R100 | R101 | R100 | R104 | R139 | R137 |
|
Isocitrate Binding |
R129 | R119 | R136 | R131 | R110 | R110 | R109 | R110 | R109 | R114 | R145 | R143 |
|
Isocitrate Binding |
R153 | R143 | R159 | R155 | R133 | R133 | R132 | R133 | R132 | R138 | R547 | R543 |
|
Isocitrate Binding |
Y160 | Y150 | Y166 | Y162 | Y140 | Y140 | Y138 | Y140 | Y139 | Y143 | Y420 | Y416 |
| Active site. | K230' | K220' | K233' | K232' | K213' | K213' | K212' | K212' | K208' | K191' | K255 | K253 |
| Active site. | D283' | D286' | D287' | D285' | D252' | D252' | D252' | D252' | D247' | D224' | D350 | D348 |
| Metal binding | D307' | D310' | D311' | D309' | D276' | D276' | D275' | D275' | D270' | D248' | D548 | D544 |
| N-loop start. | G340 | G345 | G344 | G342 | G311 | G311 | G310 | G310 | G304 | G281 | G583 | G579 |
| NADP binding | K344 | K349 | K348 | K346 | R315 | R315 | R314 | R314 | R308 | K285 | K588 | K584 |
| NADP binding | Y345 | Y350 | Y349 | Y347 | H316 | H316 | H315 | H315 | H309 | Y286 | H589 | H585 |
| N-loop end | G347 | G352 | G351 | G349 | G323 | G323 | G322 | G322 | G316 | G288 | G597 | N593 |
| N-loop extension | N352 | N357 | N356 | N354 | N329 | N329 | N328 | N328 | N322 | N293 | D602 | D598 |
Active site residues in Isocitrate Dehydrogenases. The residues in S1, S2 and S4 align properly in structural alignment. Functionally monomeric IDHs (type M) are also included for comparison. In monomeric IDHs, the respective residues don't appear in the same sequence. They do not have a Phos loop. Serine residues (such as S86 in AvIDH) play a similar role to threonines in dimeric IDHs and are indicated in italic font. N-loop refers to the NADP binding loop.