Figure 3.

Scheme for preparation of GroEL substrate. The scheme shows the logical pathway followed as one moves from selecting protein candidates that are reported as poor substrates of GroEL in a previous study. The hydrophobic patch in the protein sequence, similar to GroES mobile loop region were taken under consideration followed by computational mutation to determine their properties (GRAVY value and aggregation propensity) and energies, which made it possible to select best mutant substrates that can have appreciable binding tendency as well as proper stability.