Table 2.
Mutant Library Generated for ALLA_ECOLI and HCHA_ECOLI proteins of E.coli. The table shows a list of possible double mutants for wild type proteins and corresponding GRAVY values
| S.No. | Swissprot ID's | Suggested Patches for Mutation (similar to GGIVLTG) | Patches after mutation | GRAVY value of mutated patch* (Compare with that of GroES mobile loop)** |
|---|---|---|---|---|
| 1 | ALLA_ECOLI | GDVIETQ | GIVIITQ | 1.871 |
| ALLA_ECOLI | GDVIETQ | GIVILTQ | 1.771 | |
| ALLA_ECOLI | GDVIETQ | GIVIVTQ | 1.828 | |
| ALLA_ECOLI | GDVIETQ | GLVIITQ | 1.771 | |
| ALLA_ECOLI | GDVIETQ | GLVILTQ | 1.671 | |
| ALLA_ECOLI | GDVIETQ | GLVIVTQ | 1.728 | |
| ALLA_ECOLI | GDVIETQ | GVVIITQ | 1.828 | |
| ALLA_ECOLI | GDVIETQ | GVVILTQ | 1.728 | |
| ALLA_ECOLI | GDVIETQ | GVVIVTQ | 1.785 | |
| 2 | HCHA_ECOLI | GKLFSTG | GILFITG | 2.014 |
| HCHA_ECOLI | GKLFSTG | GILFLTG | 1.914 | |
| HCHA_ECOLI | GKLFSTG | GILFVTG | 1.971 | |
| HCHA_ECOLI | GKLFSTG | GLLFITG | 1.914 | |
| HCHA_ECOLI | GKLFSTG | GLLFLTG | 1.814 | |
| HCHA_ECOLI | GKLFSTG | GLLFVTG | 1.871 | |
| HCHA_ECOLI | GKLFSTG | GVLFITG | 1.971 | |
| HCHA_ECOLI | GKLFSTG | GVLFLTG | 1.871 | |
| HCHA_ECOLI | GKLFSTG | GVLFVTG | 1.928 |
As the single mutations for the patch doesn't make much difference in GRAVY value, so double mutations were considered.
*The GRAVY values are calculated by using ProtParam tool (ExPasy)
**The GRAVY value for mobile loop is = 1.514