Fig. 6.
(a) Example spectrum for the enzyme activity assay within the suspended-core MOF, measured 30 minutes after the first scan for the 100 U/ml sample. The black (upper) curve indicates the raw spectrum, the red (middle) curve is the reference spectrum for the substrate background (first scan, Eq. (1), the blue (lower) curve is the AMC reference spectrum (final scan, Eq. (2), and the triangles show that the determined values for βsub and βamc in Eq. (3) yield the correct spectrum. (b) The enzyme activity measured for a filled SCF where the background signal from the substrate has been subtracted.