Table 5.
Locus name | SSR repeat motif | SSR locus length (bp) | Obtained allele size range (bp) | Allele number | He | Ho | PIC value |
---|---|---|---|---|---|---|---|
MA41 | AG | 27 | 350–390 | 8 | 0.83 | 0.63 | 0.81 |
MA45 | TCTT | 16 | 110–120 | 3 | 0.6 | 0.88 | 0.53 |
MA47 | CTC | 16 | 140–160 | 2 | 0.48 | 0.81 | 0.37 |
MA412 | GAA | 25 | 350–360 | 3 | 0.67 | 0.57 | 0.59 |
MA413 | CTC | 16 | 240–250 | 3 | 0.55 | 0.42 | 0.48 |
MA418 | GAG | 13 | 320–330 | 4 | 0.7 | 0.61 | 0.64 |
MA425 | AG | 21 | 220–250 | 7 | 0.79 | 0.79 | 0.76 |
MA426 | TAAT | 14 | 150–160 | 3 | 0.27 | 0.22 | 0.25 |
MA428 | AAG | 36 | 200–220 | 4 | 0.64 | 0.33 | 0.57 |
MA432 | CAC | 13 | 300–320 | 5 | 0.67 | 0.76 | 0.61 |
MA435 | GAA | 19 | 330–350 | 4 | 0.71 | 0.83 | 0.66 |
MA440 | TTC | 19 | 170–175 | 2 | 0.19 | 0.13 | 0.17 |
MA441 | CT | 13 | 100–110 | 5 | 0.63 | 0.52 | 0.58 |
MA443 | TTC | 18 | 310–340 | 5 | 0.71 | 0.58 | 0.67 |
MA444 | CT | 33 | 260–280 | 3 | 0.29 | 0.35 | 0.26 |
MA446 | TC | 14 | 140–150 | 3 | 0.61 | 0.27 | 0.53 |
MA452 | AGA | 21 | 170–180 | 5 | 0.7 | 0.73 | 0.66 |
MA453 | CGC | 12 | 330–335 | 2 | 0.50 | 0.21 | 0.37 |
MA455 | GAA | 20 | 240–250 | 3 | 0.54 | 0.23 | 0.45 |
MA466 | CAG | 16 | 170–180 | 4 | 0.75 | 0.67 | 0.7 |
MA472 | GAGAG | 15 | 300 | 4 | 0.60 | 0.35 | 0.54 |
MA476 | AC | 13 | 200–210 | 4 | 0.72 | 0.58 | 0.67 |
MA479 | CCT | 13 | 340–350 | 4 | 0.3 | 0.38 | 0.26 |
MA489 | AT | 14 | 260–300 | 3 | 0.44 | 0.23 | 0.37 |
MA490 | CAC | 16 | 190–200 | 3 | 0.57 | 0.47 | 0.49 |
MA492 | AT | 23 | 175–180 | 5 | 0.69 | 0.75 | 0.65 |
MA493 | CTC | 12 | 310–320 | 2 | 0.5 | 0.5 | 0.37 |
MA495 | GAA | 16 | 180–220 | 2 | 0.42 | 0.00 | 0.33 |
MA4100 | AG | 14 | 320–335 | 3 | 0.38 | 0.48 | 0.33 |
MA4104 | GA | 47 | 300–320 | 4 | 0.61 | 0.38 | 0.55 |
MA4110 | TCT | 30 | 280–300 | 6 | 0.74 | 0.25 | 0.70 |
MA4111 | AGC | 26 | 375–290 | 4 | 0.73 | 0.52 | 0.68 |
MA4116 | CT | 23 | 380–410 | 5 | 0.72 | 0.65 | 0.68 |
MA4128 | CTT | 25 | 210–220 | 3 | 0.47 | 0.47 | 0.38 |
MACV11 | CAG | 20 | 210–220 | 4 | 0.5 | 0.5 | 0.46 |
MACV15 | GA | 22 | 270–290 | 6 | 0.81 | 0.33 | 0.79 |
MACV20 | TC | 12 | 340–360 | 5 | 0.72 | 0.83 | 0.66 |
MACV21 | TCA | 36 | 340–360 | 4 | 0.47 | 0.57 | 0.43 |
MACV27 | TC | 36 | 290–310 | 5 | 0.73 | 0.63 | 0.68 |
MACV29 | TAAT | 14 | 140–150 | 2 | 0.39 | 0.13 | 0.32 |
MACV36 | CT | 33 | 240–270 | 5 | 0.78 | 0.77 | 0.74 |
MACV37 | AGC | 21 | 100–110 | 3 | 0.1 | 0.1 | 0.09 |
MACV42 | AGA | 24 | 320–330 | 3 | 0.56 | 0.25 | 0.48 |
MACV47 | GA | 32 | 320–320 | 3 | 0.64 | 0.61 | 0.56 |
MACV49 | AGGCGA | 21 | 270–275 | 2 | 0.39 | 0.33 | 0.31 |
MACV50 | TC | 13 | 130–140 | 3 | 0.1 | 0.1 | 0.09 |
MACV51 | CT | 19 | 180–200 | 4 | 0.4 | 0.48 | 0.37 |
MACV54 | CAG | 12 | 160–180 | 4 | 0.68 | 0.7 | 0.62 |
MACV55 | AGA | 18 | 170–190 | 6 | 0.73 | 0.83 | 0.69 |
MACV62 | TTTTTA | 28 | 250–280 | 3 | 0.53 | 0.1 | 0.43 |
MACV63 | TGC | 25 | 120–140 | 6 | 0.72 | 0.67 | 0.67 |
MACV73 | CTCTC | 16 | 220–225 | 2 | 0.41 | 0.42 | 0.33 |
MACV77 | GCC | 12 | 90–115 | 4 | 0.58 | 0.54 | 0.52 |
MACV81 | GAA | 15 | 200–205 | 2 | 0.28 | 0.05 | 0.24 |
MACV87 | TCC | 15 | 110–130 | 4 | 0.69 | 0.83 | 0.64 |
MACV88 | GAA | 27 | 250–300 | 5 | 0.76 | 0.71 | 0.72 |
MACV96 | TCT | 15 | 180–210 | 5 | 0.75 | 0.57 | 0.70 |
MACV99 | GA | 13 | 240–260 | 2 | 0.08 | 0.08 | 0.08 |
MACV104 | AG | 14 | 290–330 | 7 | 0.81 | 0.65 | 0.79 |
MACV109 | GGA | 23 | 385–390 | 2 | 0.08 | 0.00 | 0.08 |
MACV111 | CTACA | 16 | 130–150 | 2 | 0.63 | 0.55 | 0.60 |
MACV112 | CTC | 12 | 330–360 | 3 | 0.78 | 0.74 | 0.74 |
MACV115 | AAG | 12 | 150–165 | 6 | 0.59 | 0.55 | 0.51 |
MACV128 | ATGCTC | 20 | 420–450 | 4 | 0.43 | 0.27 | 0.39 |
MACV132 | CTC | 31 | 280–290 | 4 | 0.58 | 0.65 | 0.53 |
MACV134 | CCT | 13 | 320–335 | 3 | 0.51 | 0.78 | 0.42 |
MACV139 | GA | 17 | 90–120 | 7 | 0.75 | 0.95 | 0.71 |
MACV148 | CTC | 19 | 150–170 | 5 | 0.69 | 0.57 | 0.63 |
MACV151 | TTC | 21 | 270–290 | 5 | 0.75 | 0.87 | 0.71 |
MACV154 | AAG | 12 | 130–150 | 3 | 0.12 | 0.13 | 0.12 |
MACV155 | TC | 21 | 330–350 | 3 | 0.63 | 0.26 | 0.56 |
MACV157 | GGA | 13 | 140–155 | 2 | 0.29 | 0.35 | 0.25 |
MACV161 | GCA | 12 | 110–120 | 3 | 0.61 | 0.42 | 0.53 |
MACV162 | ATCTG | 15 | 190–200 | 2 | 0.15 | 0.17 | 0.14 |
MACV169 | TC | 37 | 150–170 | 6 | 0.79 | 0.75 | 0.76 |
Polymorphism was evaluated across 20 M. acuminata accessions.
HE, expected heterozygosity under Hardy–Weinberg expectations; HO, observed heterozygosity; PIC, polymorphism information content.