Table 1.

PCR conditions for each gene.

PCR stage	IL-6	TNF	IL-1
Initial denaturation	94°C 10 min	94°C 3 min	94°C 4 min
Denaturation	94°C 1 min	94°C 1 min	94°C 30 s
Annealing	55°C 35 s	60°C 1 min	63°C 30 s
Elongation	72°C 1 min	72°C 1 min	72°C 30 s
Cycles number	35	36	35
Final elongation	72°C 10 min	72°C 5 min	72°C 10 min