Figure 1.

Tas2r gene expression in testis. (A) Reverse-transcription PCR covering nearly full coding sequences was performed for 9 Tas2r genes with cDNA templates reverse transcribed from mouse testis poly(A)⁺ RNA in the presence (+) or absence (−) of reverse transcriptases. The identities of the amplified products were confirmed by sequence analysis. (B) Quantitative real-time PCR was conducted with cDNA prepared from mouse testis poly(A)⁺ RNA. Transcripts for all the 35 known Tas2rs were detected. An average transcript copy number per nanogram of poly(A)⁺ RNA was obtained from three independent experiments with three mice. Data are means ± SEM.