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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Neurotoxicology. 2012 Feb 9;33(3):272–279. doi: 10.1016/j.neuro.2012.01.015

Figure 1. Keap1 knockdown upregulates of ARE-hPAP activity in vitro in a time-dependent and siKeap1 dependent manner.

Figure 1

A) Confluent astrocytes were treated with siKeap1 for 24 hours followed by media change and ARE-hPAP activity levels were assayed at timepoints from 1-4 days and compared to untreated cell control. B) Four different siRNA sequences all unique to Keap1 (siKeap1 1-4) were administered for 24 hours and tested for hPAP reporter activity in confluent astrocyte culture 4 days post administration. hPAP activity of tBHQ after 24 hours administration was used as a positive control. n=6; error bars SEM; * p<0.05, siKeap1 vs. siNT (black bars) and tBHQ vs vehicle (white bars).