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pH-dependency of the sulfating activity of the zebrafish SULT1 ST9 (A), SULT3 ST4 (B) and SULT3 ST5 (C) with endogeneous (17β-estradiol or DHEA) and xenobiotic (β-naphthol) compounds as substrates. The enzymatic assays were carried out under standard assay conditions as described in Materials and Methods, using different buffer systems as indicated. The data represent calculated mean ± SD derived from three experiments.
