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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Aquat Toxicol. 2012 Jan 30;112-113:11–18. doi: 10.1016/j.aquatox.2012.01.015

Table 1. Oligonucleotide primers used in the cloning and RT-PCR analysis of zebrafish SULT1 ST9, SULT3 ST4, and SULT3 ST5.

Target Sequence Sense and Antisense Oligonucleotide Primers
SULT1 ST9 Sense: 5′-CGCGGATCCATGGAAATCCAAGGCAAATCCTCTACTGATTTA-3′
Antisense: 5′-CGCGGATCCTCACTCAGTAGGGAACTTGAGAGTGGAATTCT-3′
SULT3 ST4 Sense: 5′-CGCGGATCCATGGCTCAAGAGGAATGCAAAATGATTAGTGAC-3′
Antisense: 5′-CGCGGATCCTCAACTATGCAGTTCTGTGATGTCCCAGACCAG-3′
SULT3 ST5 Sense: 5′-CGCGGATCCATGGCTCAGGAGGAATGCAAAATGATTAGTGAC-3′
Antisense: 5′-CGCGGATCCTCAGCCACGCAGTTCTGTGATGTCCCAGACCAG-3′
β-Actin Sense: 5′-ATGGATGAGGAAATCGCTGCCCTGGTC-3′
Antisense: 5′- TTAGAAGCACTTCCTGTGAACGATGGA-3′
*

Recognition sites of Bam HI restriction endonuclease in the oligonucleotides are underlined. Initiation and termination codons for translation are in bold type.

**

The sense and antisense oligonucleotide primer sets listed were verified by BLAST Search to be specific for the corresponding zebrafish SULT or β-actin nucleotide sequence.