Table 1. Oligonucleotide primers used in the cloning and RT-PCR analysis of zebrafish SULT1 ST9, SULT3 ST4, and SULT3 ST5.
Target Sequence | Sense and Antisense Oligonucleotide Primers | |
---|---|---|
SULT1 ST9 | Sense: | 5′-CGCGGATCCATGGAAATCCAAGGCAAATCCTCTACTGATTTA-3′ |
Antisense: | 5′-CGCGGATCCTCACTCAGTAGGGAACTTGAGAGTGGAATTCT-3′ | |
SULT3 ST4 | Sense: | 5′-CGCGGATCCATGGCTCAAGAGGAATGCAAAATGATTAGTGAC-3′ |
Antisense: | 5′-CGCGGATCCTCAACTATGCAGTTCTGTGATGTCCCAGACCAG-3′ | |
SULT3 ST5 | Sense: | 5′-CGCGGATCCATGGCTCAGGAGGAATGCAAAATGATTAGTGAC-3′ |
Antisense: | 5′-CGCGGATCCTCAGCCACGCAGTTCTGTGATGTCCCAGACCAG-3′ | |
β-Actin | Sense: | 5′-ATGGATGAGGAAATCGCTGCCCTGGTC-3′ |
Antisense: | 5′- TTAGAAGCACTTCCTGTGAACGATGGA-3′ |
Recognition sites of Bam HI restriction endonuclease in the oligonucleotides are underlined. Initiation and termination codons for translation are in bold type.
The sense and antisense oligonucleotide primer sets listed were verified by BLAST Search to be specific for the corresponding zebrafish SULT or β-actin nucleotide sequence.