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Summary of the procedures for the expression and purification of recombinant zebrafish SULT1 ST9, SULT3 ST4 and SULT3 ST5.
| Zebrafish SULT | Vector | Induction Condition | Affinity Resin | Elution/Digestion |
|---|---|---|---|---|
| SULT1 ST9 | pMAL-c5x | 0.5 mM IPTG; | Amylose Resin | 1 mM to 50 mM maltose; |
| SULT3 ST5 | 5 hours at 37°C | 15 min at RT*) | ||
| SULT3 ST4 | pGEX-2T | 0.1 mM IPTG; | Glutathione -Sepharose | 5 U/ml bovine thrombin; |
| Overnight at RT | -Sepharose | 15 min at RT |
RT refers to room temperature.
