Supplemental Figure 4: Gene expression of fibrotic genes.

QRT-PCR performed on atrial tissue from 6 DN-MSTN TG13 and 6 wild type animals ages 16 – 26 weeks comparing expression of various fibrosis and ECM-related genes reveals no significant increase in expression between DN-MSTN TG13 and WT hearts for overall (A), or for male mice specifically (B). Male comparison between 3 DN-MSTN TG13 and 3 wild type mice. Expression of all genes normalized to HPRT-1 as endogenous control prior to comparison between groups. None of the genes examined were significantly different between groups.