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Functional analysis of the PAI-1 E-box1 and E-box2. ECs were transfected with reporter constructs bearing 3 tandem E-box1 or E-box2 sequences (see the Materials and Methods Section) cloned into the pGL-3 control vector. After transfection, cells were left untreated (control) or incubated with 10 μM quercetin. The relative luciferase activity of unstimulated HCAEC was set as 100%. Values were expressed as means ± SE of five individual experiments; *P ≤ 0.05.
