Skip to main content
. 2012 Apr 18;32(16):5374–5384. doi: 10.1523/JNEUROSCI.3517-11.2012

Figure 2.

Figure 2.

Ferritin microinjection stimulated generation of new oligodendrocytes and increased overall oligodendrocyte numbers. A–C, Single-label and merged confocal images (≤1 μm) from a vehicle (Veh) injection site in the white matter 3 d after injection. Section is labeled for oligodendrocytes (A, CC1; red), proliferating cells (B, BrdU; green), and merged image (C). No new (i.e., double-labeled) oligodendrocytes are present in this section. D–F, Single-label and merged confocal images (≤1 μm) from a 5 mg/ml ferritin injection site 3 d after injection and labeled as above. Several new oligodendrocytes (arrows) are present in this field. Double-labeled cell near top of F is shown at higher power in inset. G, H, The number of BrdU-positive oligodendrocytes (G) and the total number of oligodendrocytes (H) at 3 d were quantified in ipsilateral white matter (WM) and gray matter (GM). Data represent mean ± SEM and are expressed relative to vehicle control numbers, indicated by dotted line. *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle control. Scale bar: (in F) A–F, 20 μm.