Figure 3.

Effects of Rho inhibition at class I and class II Rho-dependent terminators. (A) BCM effects on the class I Rho-dependent terminator at the grxD locus. A statistically significant increase in transcript levels (magenta dashed brackets) between untreated (blue bars) and BCM-treated (violet bars) cells occurs at the 3′ end of the grxD gene, indicating that the grxD transcript is terminated by Rho. (B) BCM effects on two class II Rho-dependent terminators at the bgl locus. Rho terminates antisense transcripts that arise from within the bglF and blgH genes. Note that the bglH antisense transcript is essentially undetectable in untreated conditions.