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. 2012 Dec 1;26(23):2604–2620. doi: 10.1101/gad.201327.112

Figure 2.

Figure 2.

Trr/COMPASS-like core and complex-specific subunits are required for H3K4me1. (A–G) RNAi-mediated knockdown of core members of COMPASS-like complexes (A–D) and Trr complex-specific subunits (E–G) in the posterior compartment of the wing imaginal disc. GFP expression in green marks the posterior part (highlighted by a white arrow in the antibody-only channel) where the knockdown occurs. ash2-RNAi (A′) and Rbbp5-RNAi (B′) result in strongly decreased H3K4me1, whereas other core members such as Dpy-30L1 (C′) and Hcf (D′) are not required for bulk H3K4me1. RNAi-mediated knockdowns of the Trr complex-specific subunits dUTX (E′), Ptip (F′), and Ncoa6 (G′) only weakly affect bulk levels of H3K4me1. (H) Cartoon representing the implementation of bulk H3K4me1 by the Trr/LPT complex. The SET domain-containing catalytic subunit Trr and LPT are highlighted in red, core complex subunits are in green, and complex-specific subunits are in blue. Genotypes used were as follows: UAS-Dcr-2/+; en-GAL4 UAS-EGFP/UAS-ash2-RNAi (A), UAS-Dcr-2/+; en-GAL4 UAS-EGFP/UAS-Rbbp5-RNAi (B), UAS-Dcr-2/+; en-GAL4 UAS-EGFP/+; UAS-Dpy-30L1-RNAi/+ (C), UAS-Dcr-2/+; en-GAL4 UAS-EGFP/+; UAS-Hcf-RNAi/+ (D), UAS-Dcr-2/+; en-GAL4 UAS-EGFP/+; UAS-dUTX-RNAi/+(E), UAS-Dcr-2/+; en-GAL4 UAS-EGFP/+; UAS-Ptip-RNAi/+ (F), and UAS-Dcr-2/+; en-GAL4 UAS-EGFP/+; UAS-Ncoa6-RNAi/+ (G).