Figure 1.

Amyloid-like aggregates formed by secreted CsgA_ss-NM. (A) Electron micrographs of immunolabeled CsgA_ss-NM (left) and CsgA_ss-M (right) scraped cell samples. Fibrils were detected only with the CsgA_ss-NM sample. (B) Micrographs of CsgA_ss-NM scraped cell sample harvested from CR-containing agar. Extracellular material binds CR (left) and displays apple-green birefringence when viewed between crossed polarizers (right). (C) CsgA_ss-NM, but not CsgA_ss-M, scraped cell samples contain SDS-resistant aggregates that are solubilized upon boiling, as detected by filter retention using an antibody that recognizes the M domain.