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Aggregation propensity of CsgAss-NM variants. Fibril density within scraped cell samples (CsgAss-NM, CsgAss-M, or CsgAss-NMRΔ) as detected by EM (A) and the amount of SDS-resistant material as detected by filter retention (B) parallel the known amyloidogenicity of these protein variants, as does the colony color phenotype of the cells when plated on agar containing CR.
