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. 2012 Dec 13;7(12):e51829. doi: 10.1371/journal.pone.0051829

Figure 4. EMSA of ds16-tetra-stable and ds16-octa-U binding by Dicer-dsRBD.

Figure 4

EMSA of Dicer-dsRBD binding (A) ds16-tetra-stable with a Kd = 9.1±0.1 µM and (B) ds16-octa-U with a Kd = 4.7±0.1 µM. The predicted secondary structures of the RNAs are shown above the representative gels, which were run with various Dicer-dsRBD concentration (0.25–50.12 µM) binding to 0.125 nM RNA. Fraction bound, from the EMSA data, versus Dicer-dsRBD concentration was fitted using a generalized Hill cooperative model (gray line). Error bars in the plots of fraction bound as a function of total Dicer-dsRBD concentration represent the standard deviation from duplicate measurements.