Table 1. Binding Affinity of Dicer-dsRBD in Isolation for Various Length RNA Constructs by EMSA.

RNA Construct	Dissociation Constant(Kd, µM)	Hill Coefficient(n)
pre-mir-16-1	2.2±0.1	2.2±0.1
ds44	2.4±0.1	3.2±0.1
ds33	4.9±0.1	2.8±0.1
ds22	6.5±0.1	3.4±0.2
ds16	8.9±0.1	3.4±0.2
ds12	15.9±0.1	3.8±0.1