Table 2. Effect of Hairpin Structure on the Binding Affinity of Dicer-dsRBD for dsRNA.
RNA Construct | Dissociation Constant(Kd, µM) | Hill Coefficient(n) |
ds16-tetra-stable | 9.1±0.1 | 2.9±0.1 |
ds16-tetra-U | 10.7±0.1 | 4.6±0.3 |
ds16-hexa-U | 8.9±0.1 | 3.8±0.1 |
ds16-octa-U | 4.7±0.1 | 2.8±0.1 |
ds16-flank | 4.6±0.1 | 2.5±0.1 |