Figure 8. Resveratrol suppresses tumor growth in vivo by regulating miR-21.

Resveratrol suppressed tumor growth in vivo. Twelve SCID mice were divided equally into two treatment groups- vehicle and resveratrol (20 mg/kg body weight). Drugs were administered by oral gavage every other day until the end of the study. Luciferase-tagged PC-3M-MM2 cells (1×10⁶) were injected into the flank region of each mouse on day 8 (arrow) of resveratrol treatment and then monitored for tumor growth. Resveratrol treated mice showed suppressed tumor growth (A), diminished luciferase signal (B, C), and smaller tumors (D). Representative images are shown in (B) and (D), while histograms in (C) and (D) represent mean±SEM of six mice from each treatment group. E, Tumors isolated from resveratrol-treated mice showed reduced levels of miR-21 as determined by TaqMan® assay. F, Resveratrol regulates the expression of pAkt and PDCD4 in the tumor tissue. Isolated tumors from vehicle- and resveratrol-treated mice were sectioned for immunohistochemical staining for pAkt and PDCD4. Scale bar is 50 µm. Asterisk (*) indicates statistically significant difference (p<0.05) from vehicle-treated mice.