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. 2012 Dec 15;23(24):4679–4688. doi: 10.1091/mbc.E12-08-0620

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© 2012 Peters et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Insoluble proteins accumulate in the nonquiescent population of postmitotic yeast. (A) Postmitotic cultures that had just entered stationary phase (young) or chronologically aged in stationary phase for 48 h (aged) were separated by Percoll gradient centrifugation into quiescent (Q) and nonquiescent (NQ) populations. (B) Young cells, aged Q, and aged NQ populations were harvested and insoluble protein extracted from a standard number of cells. SDS-insoluble protein from 8 × 10⁷ cells was loaded in each lane.