FIGURE 5:

SDS-insoluble proteins accumulate in autophagy-deficient cells. Total protein staining of SDS-insoluble protein extracted from wild-type, atg1Δ, or atg7Δ cells growing logarithmically in synthetic complete media (Log) or after 8 h of nitrogen starvation (SC-N). Each lane contains extract from 8 × 10⁷ cells.