FIGURE 6:

TORC1 inhibition promotes insoluble protein accumulation. (A) Total protein staining of SDS-insoluble protein extracted from 8 × 10⁷ wild-type and tor1Δ cells that had just entered the postmitotic state (young) and those postmitotically aged for 48 h (aged). (B) Total protein staining of insoluble protein extracted from 8 × 10⁷ wild-type cells growing logarithmically (Log), cells treated with either vehicle for 4 h (DMSO) or vehicle and rapamycin for 4 h (Rapa). (C) Proposed model of how the TOR pathway controls insoluble protein sequestration and autophagic pathway activation via distinct but parallel mechanisms.