FIGURE 4:

Cen2 behaves differently from Cen1. (A) Immunofluorescence images of cycling cells, showing that new (arrowhead) and old (arrow) basal bodies have equal levels of Cen1, while old basal bodies have higher levels of Cen2 than do new basal bodies. The plot shows the fluorescence ratio for the new and old basal body pairs vs. their distance. (B) Basal bodies in cells arrested by starvation have equal levels of Cen1 and Cen2, because all basal bodies are old (or mature). (A and B) Width of insets: 2.5 μm. Red, Cen1 (labels all basal bodies); green, K-like antigen (labels old basal bodies); AU, arbitrary units. (C) The CTD, but not the NTD, of Cen1 and Cen2 localize to basal bodies. Scale bars: 10 μm.