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. 2012 Dec 15;23(24):4766–4777. doi: 10.1091/mbc.E12-06-0454

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© 2012 Vonderfecht et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — The Cen1-Cen2 chimera can rescue the loss of basal bodies in the cen2Δ. (A) Immunofluorescence images showing that only the Cen1-Cen2 chimera, not the Cen2-Cen1 chimera, can rescue the cen2Δ basal body phenotypes at 30°C. (B) Immunofluorescence images showing that the Cen1-Cen2 chimera does not rescue the basal body orientation defects (arrow) in the cen2Δ at 38°C. (A and B) Green, Cen1; red, KD fibers. Scale bar: 10 μm; width of insets: 10 μm. Percentages indicate the frequency of observed phenotype for 100 cells. (C) Cen2’s domains have separate functions.

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