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. 2012 Dec 15;23(24):4807–4819. doi: 10.1091/mbc.E12-07-0555

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© 2012 Gad et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — FILIP1, but not WHAMM, is an FLNa-binding protein. (A) Endogenous FLNa from lysed HEK293T cells was precipitated using FILIP1/775-1213 and GST-WHAMM/589-809 in GST pull-down assays. The presence of FLNa in the precipitated material was revealed by Western blotting, using anti-FLNa antibodies. (B–D) Colocalization between FILIP1 or WHAMM and FLNa was revealed by transiently transfecting FLAG-tagged FILIP1 or WHAMM in COS1 cells. FLAG-tagged FILIP1 or WHAMM was visualized with mouse monoclonal anti-FLAG antibodies followed by Alexa Fluor 488–conjugated anti-mouse antibodies. FLNa was visualized using a rabbit anti-FLNa antibody, followed by TRITC-conjugated anti-rabbit antibodies. Scale bar: 20 μm.