Table 1. Lead peptides for the molecular evolution process identified from a panel of alleged G_M1-binding peptides.

No.	Lead sequences L	Fitness	Lead sequences D	Fitness
1	LPRHRDTGILDSIGRF	34,68	apqrlqwfagplrrfd	157,50
2	DFRRLPGAFWQLRQPA	31,36	tnhyekifsyteslag	102,36
3	PQIAMFCGRLNMNMNV	27,93	drhrplfghrahdmts	90,77
4	GALSETYSFIKEYHNT	26,06	plfghrahdmtsatal	87,02
5	YEVNWKTHEIKVKGQN	25,81	gtdrhrplfghrahdm	82,55
6	AEPQIAMFCGRLNMHM	25,12	ligtdrhrplfghrah	77,87
7	HHCSILKEVWHVKKLG	24,71	fikdnlthiqtnhyea	75,51
8	GWWYKGRARPVSAVAA	24,04	hrplfghrahdmtsat	64,35
9	AKVEKLCVWNNKTPHA	22,65	frgisdligtdrhrpl	59,27
10	ASDWYDEMLTWNIHGA	22,32	mhmnlrgcfmaiqpea	53,31
11	VWRLLAPPFSNRLLPA	21,46	apllrnsfppallrwv	52,06
12			vnmhmnlrgcfmaiqp	51,71
13			lrgcfmaiqpeallga	50,05

Fitness values correspond to fluorescence intensities obtained in a solid-phase binding assay with lysoG_M1/DY650-conjugate (arbitrary units) and reflect the probe-binding strength of each peptide. Highlighted amino acids were identified in “alanine walk” experiments as beneficial (boldface) or detrimental (underlined) for binding.