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. 2012 Dec 13;8(12):e1003115. doi: 10.1371/journal.pgen.1003115

Table 3. Overexpression of sli-1 reverses the effect of sli-1(sy143) on engulfment in ced-10(n1993); sli-1(sy143) animals.

Transgene Heat Shock Corpses ± s.d. n p-Value
Phspgfp 17.3±6.1 25 >0.2
Phspgfp + 16.4±6.7 23
Phspsli-1wt 13.4±4.2 34 <1×10−4
Phspsli-1wt + 23.7±6.2 27
Phspsli-1ΔRING 13.9±4.7 20 <0.006
Phspsli-1ΔRING + 20.0±6.9 15

ced-10(n1993); sli-1(sy143) embryos containing the transgenes indicated above were heat shocked at 33°C for 1 hour and then allowed to recover for 3–3.5 hours at 20°C. The numbers of cell corpses in the heads of anaesthetized first larval stage (L1) worms were counted using DIC microscopy within 0.5 h of hatching. The number of persistent corpses was determined from two independent transgenic lines for each transgene except for Phspsli-1ΔRING (see text for explanation). s.d., standard deviation.