Table 3. Overexpression of sli-1 reverses the effect of sli-1(sy143) on engulfment in ced-10(n1993); sli-1(sy143) animals.
Transgene | Heat Shock | Corpses ± s.d. | n | p-Value |
Phspgfp | − | 17.3±6.1 | 25 | >0.2 |
Phspgfp | + | 16.4±6.7 | 23 | |
Phspsli-1wt | − | 13.4±4.2 | 34 | <1×10−4 |
Phspsli-1wt | + | 23.7±6.2 | 27 | |
Phspsli-1ΔRING | − | 13.9±4.7 | 20 | <0.006 |
Phspsli-1ΔRING | + | 20.0±6.9 | 15 |
ced-10(n1993); sli-1(sy143) embryos containing the transgenes indicated above were heat shocked at 33°C for 1 hour and then allowed to recover for 3–3.5 hours at 20°C. The numbers of cell corpses in the heads of anaesthetized first larval stage (L1) worms were counted using DIC microscopy within 0.5 h of hatching. The number of persistent corpses was determined from two independent transgenic lines for each transgene except for Phspsli-1ΔRING (see text for explanation). s.d., standard deviation.