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. 2012 Dec 13;6(12):e1951. doi: 10.1371/journal.pntd.0001951

Figure 1. Effect of BZL on transporter expression in cellular lysates.

Figure 1

P-gp (panel A) and MRP2 (panel B) were detected by western blotting in HepG2 total lysates after 48 h of treatment with BZL (2, 20, 200 and 1000 µM) or vehicle (C). Equal amounts of total protein (15 µg) were loaded in the gels. MRP2 and P-gp O.D. were related to β-actin O.D. Uniformity of loading and transfer from gel to PVDF membrane was also controlled with Ponceau S. The data on O.D. (% of C) are presented as mean ± S.D. (n = 3). Typical western blot detections are shown at the bottom. *Significantly different from C, p<0.05; **Significantly different from C, p<0.01; ***Significantly different from C, p<0.001.