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. 2012 Dec 13;7(12):e51831. doi: 10.1371/journal.pone.0051831

Figure 1. Infection of S2 cells with different Leishmania species and life cycle stages.

Figure 1

A. Percentage of S2 cells infected after incubation with either L. donovani amastigotes or L. major late stationary phase promastigotes over a 24 hr time course. B. Comparison of infection levels of S2 cells and mammalian RAW264.7 macrophages incubated with late stationary phase L. major promastigotes. p = 0.0025; error bars in A and B indicate one standard error (SE). C. S2 cells infected with L. donovani amastigotes for 24 hr are stained with 1 µg/ml DAPI, 20 µg/ml propidium iodide; parasites were pre-labelled with 10 µM CFSE. D. S2 cells infected with L. donovani amastigotes (24 hr post infection) stained with anti-ARL8, a late endosomal/lysosomal marker. E. S2 cells expressing LAMP-GFP (green) infected with L. donovani amastigotes for 12 hr. C – E, scale bars, 10 µm. Arrows indicate intracellular parasites.