Figure 5. RNAi against Cdc42 reduces phagocytosis of L. donovani amastigotes.

S2 cells were treated with dsRNA against Cdc42 and Rab5 for 4 days and then incubated with amastigotes for 24 hr. A. Three wells from a 96 well plate were analysed for each treatment and at least 100 cells counted in each well. * p<0.05. B. Representative confocal laser scanning microscopy images of cells treated with dsRNA as indicated. Cells were stained with 1 µg/ml DAPI, 20 µg/ml propidium iodide; amastigotes were stained with CFSE; size bar, 10 µm.