Table 1. Classification of morphologic characteristics of colonies generated from human fibroblasts using four retroviral vectors encoding Oct3/4, Sox2, Klf4, and c-Myc.
number of nucleolus | nucleus to nucleolus ratio | cell size | cell density/mm2 | nucleus to cytoplasm ratio | number of layer | number of colonies obtained (Experiment 1) | number of colonies obtained (Experiment 2) | |
Col A | 2.35±0.51 | 3.30±0.48 | 87.69±11.04 | 2166.67±29.21 | 0.59±0.07 | multilayer | 16 | 22 |
Col B | 1.46±0.59 | 2.95±0.41 | 113.20±13.40 | 1863.06±22.49 | 0.53±0.06 | multilayer | 55 | 49 |
Col C | 1.34±0.55 | 2.50±0.50 | 69.82±9.29 | 3048.96±26.14 | 0.60±0.07 | multilayer | 95 | 102 |
Col D | 1.33±0.47 | 3.17±0.50 | 52.09±7.69 | 5091.67±21.65 | 0.6±0.05 | multilayer | 6 | 8 |
Col E | 1.24±0.48 | 2.52±0.43 | 45.72±7.14 | 3493.33±24.03 | 0.59±0.07 | multilayer | 10 | 8 |
Col F | 1.27±0.49 | 2.42±0.23 | 58.34±5.92 | 5266.67±41.01 | 0.64±0.06 | multilayer | 8 | 12 |
Col G (iPS cell) | 1.20±0.45 | 2.19±0.17 | 43.50±2.96 | 5933.33±20.82 | 0.87±0.04 | single | 2 | 2 |
human ES cell | 1.20±0.45 | 2.20±0.17 | 43.70±3.61 | 6133.33±15.32 | 0.86±0.04 | single |
Each replicate represented 2×105 transduced cells seeded onto a 60-mm dish containing feeder cells and cultured in Primate ES cell medium for thirty days.