Table 2. Classification of morphologic characteristics in colonies generated from human fibroblasts using a single polycistronic viral vector.

	number of nucleolus	nucleus to nucleolus ratio	cell size	cell density/mm2	nucleus to cytoplasm ratio	number of layer	number of colonies obtained (Experiment 1)	number of colonies obtained (Experiment 2)
Col H	1.30±0.50	3.60±0.60	87.50±9.36	1933.34±19.05	0.58±0.06	single	16	11
Col I	1.33±0.54	2.71±0.46	70.01±7.13	2962.96±20.91	0.56±0.05	single	64	66
Col J	1.26±0.47	2.65±0.45	47.09±7.34	3745.00±24.86	0.61±0.07	multilayer	71	69
Col K	1.20±0.45	2.50±0.49	65.06±5.69	5466.67±40.92	0.55±0.05	single	16	23
Col L	1.25±0.47	2.48±0.39	51.55±5.91	5383.84±34.67	0.59±0.06	multilayer	30	21
Col G (iPS cell)	1.20±0.45	2.19±0.17	43.50±2.96	5933.33±20.82	0.87±0.04	single	2	2

Each replicate represented 2×10⁵ GFP positive cells seeded onto a 60-mm dish containing feeder cells and cultured in Primate ES cell medium for thirty days.