Table 2. Classification of morphologic characteristics in colonies generated from human fibroblasts using a single polycistronic viral vector.
number of nucleolus | nucleus to nucleolus ratio | cell size | cell density/mm2 | nucleus to cytoplasm ratio | number of layer | number of colonies obtained (Experiment 1) | number of colonies obtained (Experiment 2) | |
Col H | 1.30±0.50 | 3.60±0.60 | 87.50±9.36 | 1933.34±19.05 | 0.58±0.06 | single | 16 | 11 |
Col I | 1.33±0.54 | 2.71±0.46 | 70.01±7.13 | 2962.96±20.91 | 0.56±0.05 | single | 64 | 66 |
Col J | 1.26±0.47 | 2.65±0.45 | 47.09±7.34 | 3745.00±24.86 | 0.61±0.07 | multilayer | 71 | 69 |
Col K | 1.20±0.45 | 2.50±0.49 | 65.06±5.69 | 5466.67±40.92 | 0.55±0.05 | single | 16 | 23 |
Col L | 1.25±0.47 | 2.48±0.39 | 51.55±5.91 | 5383.84±34.67 | 0.59±0.06 | multilayer | 30 | 21 |
Col G (iPS cell) | 1.20±0.45 | 2.19±0.17 | 43.50±2.96 | 5933.33±20.82 | 0.87±0.04 | single | 2 | 2 |
Each replicate represented 2×105 GFP positive cells seeded onto a 60-mm dish containing feeder cells and cultured in Primate ES cell medium for thirty days.