Figure 3. The effect of diet induced obesity in C57BL6 mice on Acetylation and DNA damage levels in spermatids during protanimation. Data taken from.³⁵ Data was analyzed through a univariate general linear model with replicate fitted as a covariate and mouse ID as a random factor. Correlation data was determined by a Pearson’s Rho. (A) The effect of diet induced obesity in mice on acetylation levels of H3K9 in elongating spermatids representative of > 120 spermatids from at least 5 mice per treatment group. (B) The effect of diet induced obesity in mice on DNA damage levels in elongating spermatids representative of > 4000 spermatids from at least 5 mice per treatment group. There was a negative correlation found between acetylation levels of H3K9 and DNA damage levels in round spermatids R² = 0.60, p = 0.023 n = 8 animals.