Table 3.
Statistically significant differences in CLL cell viability between different CLL subgroups with distinct clonotypic BcRs after stimulation of TLRs and NOD2 with their respective ligands.a
| Evaluated TLR | Time point | Compared groups | Median values, % | p |
|---|---|---|---|---|
| TLR1/2 | d 9 | M versus UM | 16.9 versus −3.6 | 0.006 |
| #4 versus #1 | 44.3 versus −1.7 | 0.003 | ||
| M versus #4 | 16.9 versus 44.3 | 0.029 | ||
| TLR2/6 | d 9 | M versus UM | 17.1 versus −8.1 | 0.001 |
| #4 versus #1 | 42.2 versus −8.1 | 0.001 | ||
| #4 versus non-#4/16 | 42.2 versus 6.8 | 0.050 | ||
| M versus #4 | 17.1 versus 42.2 | 0.026 | ||
| TLR7 | d 3 | M versus UM | −21.5 versus 1.2 | 0.007 |
| d 6 | M versus UM | −27.3 versus −7.3 | 0.007 | |
| #4 versus non-#4/16 | −18 versus −43.2 | 0.050 | ||
| TLR9 | d 3 | #4 versus #1 | −7.5 versus 23.7 | 0.031 |
| UM versus #1 | 7.7 versus 23.7 | 0.050 | ||
| d 6 | M versus UM | −16.7 versus 1.7 | 0.006 | |
| NOD2 | d 3 | UM versus #1 | 5.5 versus 18.3 | 0.009 |
| d 9 | #4 versus #1 | 36.6 versus 16.6 | 0.016 |
a
Median values represent the percentage of live cells (annexin V−/PI−). Before statistical analysis values were normalized to the unstimulated controls.
M, mutated; UM, unmutated.