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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Immunother. 2013 Jan;36(1):3–10. doi: 10.1097/CJI.0b013e3182791234

FIGURE 4. Immunomodulatory cytokines do not affect transgene expression in piggyBac-transposed human T cells.

FIGURE 4

Five representative clones (C1, C2, C3, C8, and C19) were stimulated on anti-CD3/CD28 antibody coated plates for 4 days and then cultured for 35 days. Stimulated clones were classified into 3 different groups based on the activation state such as “actively dividing”, “activated”, and “resting”. Those in “actively dividing” received cytokines on day 1, “activated” received cytokines on day 5, and “resting” received cytokines on day 21. Clones were treated with interferon-gamma (IFN-γ) (10 ng/mL), IL-12 (10 ngmL), IL-4 (1,000 IU/mL) or transforming growth factor-beta (TGF-β) (5 ng/mL) for 4 days. Four days after the treatment, the clones were analyzed for GFP expression by flow cytometry.