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. 2012 Nov 15;5:637. doi: 10.1186/1756-0500-5-637

Table 2.

In vitro analysis of primer specificity of the primers used in this study

Target Probe Annealing temp(°C) No. of target strains detecteda Sensitivity (%)b No. of non-target strains detecteda Specificity (%)b Reference
G. vaginalis
 Gard154-454
 60
 3 (from 3)
 100.0
 0 (from 6)
 100.0
 This study
 
  
 62
 12 (from 12)
 100.0
 0 (from 34)
 100.0
  
 
 GV1 + 3
 60
 3(from 3)
 100.0
 0 (from 6)
 100.0
 [15]
 
  
 62
 10 (from 12)
 83.3
 0 (from 34)
 100.0
  
 
 Gv1 + 2
 60
 3 (from 3)
 100.0
 0 (from 6)
 100.0
 [13]
 
  
 62
 11 (from 12)
 91.7
 0 (from 34)
 100.0
  
A. vaginae
 Atop109-329
 60
 3 (from 3)
 100.0
 5 (from 6)
 16.7
 This study
 
  
 62
 12 (from 12)
 100.0
 15 (from 24)
 37.5
  
 
  
 66
 12 (from 12)
 100.0
 3 (from 34)
 91.2
  
 
 AtovagRT3
 60
 12 (from 12)
 100.0
 1 (from 24)
 95.8
 [11]
 
  
 62
 12 (from 12)
 100.0
 2 (from 34)
 94.1
  
 
 Atop167-587
 60
 2 (from 3)
 66.7
 0 (from 6)
 100.0
 [10]
 
  
 62
 11 (from 12)
 91.6
 4 (from 34)
 88.3
  
Lactobacillus spp.
 AM Lacto
 60
 2 (from 12)
 16.7
 1 (from 24)
 95.8
 This study
 
  
 58
 2 (from 12)
 16.7
 3 (from 34)
 91.2
  
 
 New Lacto
 60
 5 (from 12)
 41.7
 18 (from 34)
 47,1
 [16]
 
  
 62
 1 (from 12)
 8.3
 8 (from 24)
 66.7
  
 
 S21 + A19
 60
 5 (from 12)
 41.7
 24 (from 24)
 0
 [17]
    62 6 (from 12) 50.0 34 (from 34) 0  

aCalculated using ProbeMatch (last accession, May 2012) with the following data set options: Strain – Both; Source – Both; Size – > 1200 bp; Quality – Both.

bFormula: Specificity = (nts/Tnts)*100 (nts: number of non-target strains undetected, Tnts: total number of non-target strains tested).

Sensitivity = (ts/Tts)*100 (ts: number of target strains detected, Tts: total number of target strains tested).