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Flow cytometry sorting of human endometrial mesenchymal stromal cells (MSC). (A) Scatterplot of human endometrial cells. (B) Electronic gating used to obtain viable (Sytox Blueneg) and exclude CD45+ leukocytes. Region R6 was then analyzed for (C) coexpression of CD140 and CD146. Double-stained CD140b+CD146+ cells in R2 gate were then sorted.
