Table 1 . Development of Am-tra2-dsRNA-treated female embryos.
| Embryos injected | No. of embryos showing normal development after ∼70 hr |
Hatched L1 larvae |
|||
|---|---|---|---|---|---|
| Treatment/amount of injected dsRNA per embryo | N | N | % | N | % |
| Nontreated | 683 | 594 | 86.9 | 365 | 53.4 |
| ddH20 | 406 | 182 | 44.8 | 97 | 23.9 |
| Am-tra2 dsRNA-1 | |||||
| 200 pg | 92 | 0 | 0 | 0 | 0 |
| 100 pg | 25 | 0 | 0 | 0 | 0 |
| 75 pg | 60 | 0 | 0 | 0 | 0 |
| 40 pg | 20 | 0 | 0 | 0 | 0 |
| 20 pg | 53 | 0 | 0 | 0 | 0 |
| 4 pg | 40 | 5 | 12.5 | 2 | 5.0 |
| Am-tra2 dsRNA-2 | |||||
| 224 pg | 27 | 0 | 0 | 0 | 0 |
| 96 pg | 100 | 15 | 15 | 0 | 0 |
| 67 pg | 104 | 38 | 36.5 | 0 | 0 |
| 56 pg | 105 | 14 | 13.3 | 4 | 3.8 |
| 33 pg | 146 | 31 | 21.2 | 6 | 4.1 |
| 4 pg | 155 | 29 | 18.7 | 9 | 5.8 |
The number of individuals (N) and the relative proportions (%) with respect to the total number of initially injected embryos are shown.