Figure 3 .

Cdx2 and Oct4 bind to Xist and Xite in vivo. (A) Map of Oct4- and Cdx2-binding motifs within Xist and Xite. The Cdx2 consensus and binding motifs within Xist intron 1, Xite, and HoxC8 (a known Cdx2 target gene) are shown. (B) qChIP analysis of Oct4 and Cdx2 binding at designated sites in the indicated cell lines. ES, Cdx2-transgenic ES clones without Tx induction of Cdx2. iTS, Cdx2-transgenic clones transdifferentiated to TS cells by Tx induction of Cdx2. TS, trophoblast stem cells. Mean ± SEM of three independent biological replicates is shown. The statistical significance of each result is calculated compared to the control IgG ChIP (background), using a paired, one-tailed Student’s t-test. *P < 0.05. (C) qRT-PCR for Oct4 RNA in the indicated cell lines. Levels are normalized to β-actin levels. Mean ± 1 SD is shown.