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Subset of samples revealing paternal mtDNA using AS-PCR1. M: 100 bp ladder; 1 and 2: two positive cases from pair #4; 3 and 4: two positive cases pair from #21; 5 and 6: two positive cases from pair #72; 7 and 8: two positive cases from pair #85; 9: no template control; 10: positive control for 2-KY0418; 11: positive control for 3-KY0410. Gender of offspring: 1, 2, 5–7: female; 3, 4, 8: male.
