Figure 1.

Experimental design for generating outcross control seeds, selfed seeds and biparentally inbred seeds. Three crosses were performed between unrelated plants that were fully or partly genotyped for the S-locus to generate F1 cross-progeny with three different parental PB. In PB1 and PB2, the cross involved self-incompatible (SI) plants from the outcrossing population from Pinery Provincial Park (PIN). In PB3, a SI plant from PIN was crossed with a self-compatible (SC) plant from the inbreeding population from Rondeau Provincial Park (RON). A subset of the resulting F1 seeds were germinated and genotyped at SRK, which is the gene controlling female specificity (expected S-locus genotypes in the F1 are indicated), the remainder were kept to serve as control outcrossed seeds. F1 that could be unambiguously genotyped for the S-locus (larger font) were manually selfed in a CO₂-rich environment. In addition, in PB3 crosses were performed between two compatible F1-sibling SRK genotypes (S₁S₂₃ × S₁S_?) to generate biparentally inbred progeny. In PB3, the S₁ haplotype derived from the inbred parent is indicated in red. A full color version of this figure is available at the Heredity journal online.