Table 2. Segregation of S-haplotypes in relation to survival to 4 weeks of age in enforced selfed progeny derived from the three parental backgrounds.
Survival |
Total |
||
---|---|---|---|
N | Y | O (E) | |
Selfed progeny PB1 (S1S39) | |||
S1S1 | 1 | 3 | 4 (10) |
S1S39 | 12 | 18 | 30 (20) |
S39S39 | 3 | 3 | 6 (10) |
Probability | 0.762a | 0.00654b | |
Selfed progeny PB2 (S3S23) | |||
S3S3 | 1 | 7 | 8 (13) |
S3S23 | 5 | 9 | 14 (26) |
S23S23 | 9 | 21 | 30 (13) |
Probability | 0.577a | 0.000003b | |
Selfed progeny PB3 (S1S23) | |||
S1S1 | 1 | 8 | 9 (10.5) |
S1S23 | 3 | 19 | 22 (21) |
S23S23 | 1 | 10 | 11 (10.5) |
Probability | 1.00a | 0.919b |
Abbreviation: PB, parental background.
To test whether there were differences in survival by genotype, we used Fisher's exact tests to compare the frequency distribution of S-locus genotypes among progeny that did not (N) and did (Y) survive to 4 weeks of age. The exact probability for the observed or a larger difference is given.
To test for overall segregation distortion, we tested for deviations from expected Mendelian ratios (1:2:1) based on observed (O) and expected (E) genotype frequencies summed across both surviving and dead progeny. The exact multinomial probability for the observed or a larger deviation is given.