FIGURE 2.

Distinct regions of JRAB MID are required for the binding of actinin-1/4 and PM targeting. A and D, HEK293 cells expressing Myc-actinin-1 or -actinin-4 were lysed and subjected to pulldown assays using GST proteins. The bound proteins were detected by immunoblotting (IB). The schema indicates each domain and its binding to actinins. B, C, E, and F, MTD-1A cells expressing GFP-tagged full-length or deletion mutants of JRAB were fixed and processed for double fluorescence. Arrows indicate PM targeting. The schema indicates each domain and its PM targeting activity. Bar, 20 μm. The results shown are representative of at least three independent experiments.